Purification and optimization of pink pigment produced by newly isolated bacterial strain Enterobacter sp. PWN1

Abstract Pigment-producing bacteria were isolated from kitchen wastewaters of the National Institute Technology, Rourkela. A pink non-virulent bacterial strain PWN1 was selected based on India Ink Broth and Coomassie Brilliant Blue (R-250) dye assay. According to morphological biochemical characterization, PWN1was a Gram-negative, rod-shaped, motile, non-coliform bacterium could utilize only glucose adonitol as sole carbon source. The pigment found be growth-associated product, production accelerated after 40 h culture. Further, 16S rRNA gene-based molecular identification showed its similarity with Enterobacter sp. pigments extracted by solvent extraction method using chloroform ethanol (3:1). then purified through thin-layer chromatography column chromatography. To maximize production, culture condition optimized for maximum biomass statistical software Design Expert v13. quadratic model structured describing process efficiently it suggested moderate temperature, pH, high inoculum concentration which generated 3.81 ± 0.02 g/L. At condition, 1 L cell produced 3.77 g crude 0.234 0.131 chromatography, implying yield 6.2% 3.47% biomass. obtained enough draw interest industrial although application is considerable further study.